U.S. Department of Agriculture (USDA) Animal and Plant Health Inspection Service. Animal Care Annual Report of Activities Fiscal Year 2007.
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This is the most recent report available.

See Appendix V for:
  • FY 2007 AWA inspections
  • Animals used in research: all categories
  • Animals used in research: no pain and no drugs
  • Animals used in research: with pain and no drugs
  • Animals used in research: with pain and with drugs
  • Number of animals used by research from the first reporting year (FY 1973) to FY 2007

United States laboratory animal use is federally regulated by the Animal Welfare Act (1966, amended 1985), which excludes laboratory-bred mice and rats, as well as non-mammals, from consideration and protection (Goldberg 2002, Stephens et al. 2002), despite the fact that mice and rats comprise the overwhelming majority of all laboratory subjects. These USDA statistics exclude unregulated species, and hence represent a tiny fraction of US laboratory animal use. For example, although 1.0 million regulated animals were used in Fiscal Year 2007, Taylor and colleagues estimated that 17.3 million living non-human vertebrates were used in 2005, making the US the leading global user. Japan was second and China third, using 11.2 and 3.0 million of these animals, respectively. Their calculations based on animal study publication rates provide the most accurate evidence-based estimation to date.

References
  • Goldberg, A.M. (2002). Use of animals in research: a science--society controversy? The American perspective: animal welfare issues. Altex: Alternatives to Animal Experimentation 19(3), 137-139.
  • Taylor K, Gordon N, Langley G & Higgins W. Estimates for worldwide laboratory animal use in 2005. Altern Lab Anim 2008; 36: 327–342.
  • Stephens, M.L., Alvino, G.M. & Branson, J.B. (2002). Animal pain and distress in vaccine testing in the United States. Developments in biologicals 111, 213-216.